Imaging and modelling 3D cell culture systems

Our latest workshop was a joint event with another Technology Touching Life network, IBIN (Integrated Biological Imaging Network) in London. This was our first joint IBIN-3DBioNet workshop where over 100 attendees from all over the UK met during two days in January. IBIN‘s focus is on developing new methods of taking high-resolution images of living cells in 3D systems and tissues. So, the complementarity with 3DbioNet is obvious. The first day included a session on mental health organised and chaired by early career researchers. The power of combining experimental and mathematical modelling approaches… Read More

Funded Project Summary:

Dr Iestyn Pope’s team at Cardiff University was recently awarded £53,950 to take the following project forward: Incorporating deuterium into 3D organoids to identify cell types and track cellular metabolic rates using CARS microscopy Organoids are 3D microtissues that reproduce more accurately how a tissue behaves compared to a 2D culture on plastic. This is because it allows the cells to interact with each other and to organise their own microenvironment. Recently, we and others have shown that patient-derived cancer organoids retain the cellular diversity that matches that of the tumours from… Read More

Pump-priming awardees confirmed:

3DbioNet received 21 eligible applications in total, and had a final ranking review call with our advisory board on Friday August 30th, the outcome of which we are pleased to share. We were only able to fund four projects in this round, there are many more we would have liked to have funded. The four successful projects are as follows: Award amount: £45,750Principal Investigator: Dr Ipsita Roy Institution: University of Sheffield, Material Science and EngineeringProject title: A natural and sustainable biomaterial-based 3D model of healthy cardiac tissue Award amount: £63,980Principal Investigator: Prof Ruth CameronInstitution: Cambridge Centre for Medical Materials /… Read More

Funded Project Summary:

Professor Ruth Cameron’s team at the University of Cambridge was recently awarded £63,980 to take the following project forward: Directing respiratory organoid development via tailored three-dimensional macromolecular environments Our aim is to produce alveolar organoid structures in highly defined 3D structures. This will allow us to transition away from current spherical organoid systems, typically cultured using Matrigel, to a 3D system that more appropriately reflects tissue organisation. We propose to design ice –templated, collagen- and elastin-based, porous environments to supply the spatial, mechanical and biochemical cues of native tissue. The self-organisation of… Read More

Funded project summary:

Dr Eirini Velliou’s team at the University of Surrey was recently awarded £57,906 to take the following project forward: On the design of a biomimetic 3D printed metastasis prototype of primary ovarian cancer – towards personalised healthcare Ovarian cancer is a silent gynecological killer with late stage diagnosis, relatively low survival rate, high resistance to chemotherapy and high recurrence rate. While research is being conducted to answer, and solve many of its associated problems, the lack of a robust, high throughput in vitro model for the disease and its metastasis puts a… Read More

Collaborative Challenge update: First Progress Report from Dr. Adedamola Olayanju

Dr. Olayanju, one of 3DbioNet’s Collaborative Challenge award recipients, has been investigating the use of PeptiGels in the development of gastro-intestinal (GI) organoids. He sends the following project update: INTRODUCTION A major challenge in advancing preclinical studies is the lack of robust in vitro culture systems that fully recapitulate what happens in vivo. Organoids, the 3-dimensional (3D) self-replicating structures are increasingly being shown to be powerful models for ex vivo experimentation in the field of tissue engineering. Organoid formation requires the use of extracellular matrix (ECM) components to form the 3D conformation…. Read More