ECR Club Kick off Social Lunch

ECR Social Lunch 3rd July 2020 12-1pm report by Jonathan Temple The Early Career Researcher (ECR) social lunch was kicked off in a virtual session by a warm welcome by Dr. Bhumika Singh, the 3DbioNet manager, who introduced the ECR events organising committee to the participants. Currently, the committee members are Dr Caroline Taylor, University of Sheffield, Jonathan Temple, University of Liverpool and Sebastian Gilbert, University of Birmingham. Caroline outlined the aims of the session, and the overall aims of the ECR club. Following a quick round of introduction from all the… Read More

Respiratory organoid development via tailored three-dimensional macromolecular environments: a 3DbioNet funded project

Professor Ruth Cameron’s team at the University of Cambridge was awarded pump priming grant by 3DbioNet. The aim of the study is to produce alveolar organoid structures in highly defined 3D structures. This will allow transitioning away from current spherical organoid systems, typically cultured using Matrigel, to a 3D system that more appropriately reflects tissue organisation. The group propose to design ice –templated, collagen- and elastin-based, porous environments to supply the spatial, mechanical and biochemical cues of native tissue.Below is a video we filmed from our trip to their laboratory in Cambridge.

CARS microscopy for imaging 3D organoids: a 3DbioNet funded project

Dr Iestyn Pope’s team at Cardiff University was awarded pump priming grant for this project titled ”Incorporating deuterium into 3D organoids to identify cell types and track cellular metabolic rates using CARS microscopy”. In this study, Dr. Pope’s group intend to use CARS microscopy to image organoids that have been cultured in media containing deuterated water. The aim being to identify the different cell types that make up the organoid structures without the need for fluorescent markers. Similarly by feeding the cells with deuterated compounds we will be able to observe the… Read More

3D model of ovarian cancer and metastasis: a 3DbioNet funded project

3DbioNet funded a range of projects, one of them was at Dr. Eirini Velliou’s group at the University of Surrey. The project aims to design a biomimetic 3D printed prototype of primary ovarian cancer.”We want to see what happens when a patient develops resistance to a certain disease” , Dr. Priyanka Gupta, Velliou’s group, University of Surrey.

Early Career Researchers Committee

3DbioNet have started an initiative to promote learning and training among the early career researchers interested in 3D biology. A committee has been set up to lead the activities, some of which would be around training in techniques, new methods, as well as career mentorship. Dr. Caroline Taylor, University of Sheffield, and Jonathan Temple, University of Liverpool have been appointed as the ECR committee members. If you would like to become a member of the ECR committee, or to contribute, please email at 3dbionet@liverpool.ac.uk.

Imaging and modelling 3D cell culture systems

Our latest workshop was a joint event with another Technology Touching Life network, IBIN (Integrated Biological Imaging Network) in London. This was our first joint IBIN-3DBioNet workshop where over 100 attendees from all over the UK met during two days in January. IBIN‘s focus is on developing new methods of taking high-resolution images of living cells in 3D systems and tissues. So, the complementarity with 3DbioNet is obvious. The first day included a session on mental health organised and chaired by early career researchers. The power of combining experimental and mathematical modelling approaches… Read More

Funded Project Summary:

Dr Iestyn Pope’s team at Cardiff University was recently awarded £53,950 to take the following project forward: Incorporating deuterium into 3D organoids to identify cell types and track cellular metabolic rates using CARS microscopy Organoids are 3D microtissues that reproduce more accurately how a tissue behaves compared to a 2D culture on plastic. This is because it allows the cells to interact with each other and to organise their own microenvironment. Recently, we and others have shown that patient-derived cancer organoids retain the cellular diversity that matches that of the tumours from… Read More

Pump-priming awardees confirmed:

3DbioNet received 21 eligible applications in total, and had a final ranking review call with our advisory board on Friday August 30th, the outcome of which we are pleased to share. We were only able to fund four projects in this round, there are many more we would have liked to have funded. The four successful projects are as follows: Award amount: £45,750Principal Investigator: Dr Ipsita Roy Institution: University of Sheffield, Material Science and EngineeringProject title: A natural and sustainable biomaterial-based 3D model of healthy cardiac tissue Award amount: £63,980Principal Investigator: Prof Ruth CameronInstitution: Cambridge Centre for Medical Materials /… Read More

Funded Project Summary:

Professor Ruth Cameron’s team at the University of Cambridge was recently awarded £63,980 to take the following project forward: Directing respiratory organoid development via tailored three-dimensional macromolecular environments Our aim is to produce alveolar organoid structures in highly defined 3D structures. This will allow us to transition away from current spherical organoid systems, typically cultured using Matrigel, to a 3D system that more appropriately reflects tissue organisation. We propose to design ice –templated, collagen- and elastin-based, porous environments to supply the spatial, mechanical and biochemical cues of native tissue. The self-organisation of… Read More

Funded project summary:

Dr Eirini Velliou’s team at the University of Surrey was recently awarded £57,906 to take the following project forward: On the design of a biomimetic 3D printed metastasis prototype of primary ovarian cancer – towards personalised healthcare Ovarian cancer is a silent gynecological killer with late stage diagnosis, relatively low survival rate, high resistance to chemotherapy and high recurrence rate. While research is being conducted to answer, and solve many of its associated problems, the lack of a robust, high throughput in vitro model for the disease and its metastasis puts a… Read More