Mini-brain organoids: a Post Card from John Hopkins
During the visit, Dr Lena Smimova showed me how to culture mini-brain organoids (spheroids) from iPS-derived NPC using a shaker protocol.
I also did one set of my own culture and follow it for 5 days. At the same time, I did immunofluorescence staining for fixed spheroids provided by Dr Smimova. It was an enjoyable and successful trip for our collaboration.
At the moment, I am setting up the culture system at Sheffield Institute for Translational Neuroscience (SITraN) and will compare it with a protocol from Professor Jens Schwamborn at University of Luxembourg by using the Quasi Vivo millifluidic system from Kirkstall Ltd.
I have contacted Simon Butterworth at Kirkstall to arrange delivery the system in the near future.
Senior Lecturer in Translational Neuroscience